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LiFluor™荧光标记染料
生物分子的荧光标记使研究人员能够在成像、流式细胞术和蛋白质印迹等应用中以极高的灵敏度检测复杂生物组装体的特定成分。 LifeSct 隶科生物提供全系列 LiFluor™ 荧光标记染料，用于标记抗体、蛋白质、肽、配体、合成寡核苷酸和其他生物分子，用于免疫化学、荧光原位杂交 (FISH)、细胞示踪、受体标记和细胞化学应用以及探测生物结构、功能和相互作用。LiFluor™ 染料具有高清明亮、高度光稳定性和优异的水溶性，涵盖可见光和近红外 (IR) 光谱，可用于标记生物分子，尤其是蛋白质和核酸。
产品优势
• LiFluor™ 荧光染料具有高清明亮和良好的光稳定性
• 对pH值变化不敏感，并且具有良好的水溶性
• 广谱性：从紫外到近红外的整个光谱
| 荧光染料 | 发光类型 | 优点 | 替代 |
| LiFluor™ 350 Blue | UV | Highly fluorescent; Highly water- soluble and pH insensitive | AMCA, Alexa Fluor 350, DyLight 350 |
| LiFluor™ 405 Blue | 405 nm laser | Brighter than Pacific Blue; Less spill-over in the green channel | Pacific Blue, BD Horizon V450 |
| LiFluor™ 405 Green | 405 nm laser | Highly fluorescent; Less spill- over in the green channel | BD Horizon V500 |
| LiFluor™ 488 Green | 488 nm laser | Extremely photostable; Highly fluorescent and water-soluble | Alexa Fluor 488, DyLight 488, Cy2, FITC |
| LiFluor™ 546 Red | 532, 543, 546 or 555 nm laser | Extremely photostable; Highly fluorescent and water-soluble | Alexa Fluor 546, Cy3, TAMRA |
| LiFluor™ 555 Red | 532, 543, 546 or 555 nm laser | Brighter than TAMRA; Highly photostable and water-soluble | Alexa Fluor 555, DyLight 549, Cy3, TAMRA |
| LiFluor™ 568 Red | 543, 546, 555 or 568 nm laser | Highly water-soluble and pH insensitive; Highly fluorescent | Alexa Fluor 568, ATTO 565, Rhodamine Red |
| LiFluor™ 594 Red | 555 or 568 nm laser | Brighter than Texas Red; Highly water-soluble and pH insensitive | Alexa Fluor 594, DyLight 594, Texas Red |
| LiFluor™ 610 Red | 555 or 568 nm laser | Highly fluorescent; Highly water- soluble and pH insensitive | Alexa Fluor 610 |
| LiFluor™ 635 Red | 633 or 635 nm laser | Highly fluorescent; Highly water- soluble and pH insensitive | Alexa Fluor 635, DyLight 633 |
| LiFluor™ 647 Red | 633 or 635 nm laser | Brighter than Cy5; Highly water- soluble and pH insensitive | Alexa Fluor 647, DyLight 649, Cy5 |
| LiFluor™ 680 Red | 680 or 685 nm laser | Brighter than Cy5.5; Highly water-soluble and pH insensitive | Alexa Fluor 680, DyLight 680, Cy5.5 |
| LiFluor™ 750 Red | 680 or 685 nm laser | Highly fluorescent; Highly water- soluble and pH insensitive | Alexa Fluor 750, DyLight 750, Cy7 |
成功案例
Comparison of relative fluorescence of goat anti-rabbit IgG antibody conjugates of LiFluor™ dyes and Cy dyes at different dye:protein ratios in the conjugate.
Comparison of the photobleaching rates of LiFluor™ 488 goat anti-mouse IgG antibody conjugate (top) with FITC goat anti-mouse IgG antibody conjugate (bottom).
