| Applications: | ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | HRP |
| Gene Name: | immunoglobulin heavy constant gamma 2 (G2m marker) |
| Gene Symbol: | IGHG2 |
| Synonyms: | IgG2 |
| Gene ID: | 3501 |
| UniProt ID: | P01859 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 99-326 of human IgG2 (Fc) (P01859). |
| Dilution: | ELISA 1:5000-1:10000 |
| Purification Method: | Affinity purification |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
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Dose response curve of HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG2 (Fc) has highly specific recognition of Human IgG2、Human IgG while no or minimal cross reactivity to Human IgG1、Human IgG4、Human IgM、Rat IgG. |