| Applications: | FC |
| Host Species: | Mouse |
| Isotype: | IgG |
| Conjugation: | APC. Ex:650nm. Em:660nm. |
| Dilution: | FC, 5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Buffer: | PBS with 0.09% Sodium azide, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
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Flow cytometry: 1X10^6 Raji cells (negative control,left) and Human PBMC (right) were surface-stained with APC Mouse anti-Human CD3 mAb (A25082,5 μl/Test,orange line) or APC Mouse IgG 2a isotype control (A26377,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
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Flow cytometry: 1X10^6 Human PBMC were surface-stained with APC Mouse IgG 2a isotype control (A26377,5 μl/Test,left) or APC Mouse anti-Human CD3 mAb (A25082,5 μl/Test,right). |