| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | ABflo® 450. Ex:406nm. Em:445nm. |
| Immunogen: | A synthesized peptide derived from rabbit IgG isotype control. |
| Dilution: | FC, 5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
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Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit anti-Human CD4 mAb (A26523,5 μl/Test,orange line) or ABflo® 450 Rabbit IgG isotype control (A27451,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
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Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (A27451,5 μl/Test,left) or ABflo® 450 Rabbit anti-Human CD4 mAb (A26523,5 μl/Test,right). |
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Flow cytometry: 1X10^6 HeLa cells (negative control,left) and THP-1 cells (right) were surface-stained with ABflo® 450 Rabbit anti-Human CD4 mAb (A26523,5 μl/Test,orange line) or ABflo® 450 Rabbit IgG isotype control (A27451,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
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Flow cytometry: 1X10^6 THP-1 cells were surface-stained with ABflo® 450 Rabbit IgG isotype control (A27451,5 μl/Test,left) or ABflo® 450 Rabbit anti-Human CD4 mAb (A26523,5 μl/Test,right). |