DNase I (5000 U)

Deoxyribonuclease I (DNase I) is an endodeoxyribonuclease that digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). It recognizes and cleaves phosphodiester bonds, yielding single deoxynucleotides or oligonucleotides (either single- or double-stranded) with a phosphate group at the 5' end and a hydroxyl group at the 3' end.

DNase I activity is Ca²⁺-dependent and can be activated by divalent metal ions such as Mg²⁺ and Mn²⁺. In the presence of Mg²⁺, DNase I randomly recognizes and cleaves any site on either strand of dsDNA. In the presence of Mn²⁺, it recognizes and cleaves nearly identical sites on both strands of dsDNA, generating blunt-end DNA fragments or sticky-end fragments with 1–2 nucleotide overhangs.
 

Key Features

• Endodeoxyribonuclease Activity: Digest both single-stranded and double-stranded DNA efficiently.

• GMP Grade: Manufactured under strict GMP-compliant production and quality management systems.

• No Antibiotics or Animal-Derived Components: Free from ampicillin and animal-derived raw materials/excipients throughout production.

• Full Traceability: Complete traceability of production process, raw materials, and auxiliaries.

• mRNA Vaccine Ready: Suitable as raw or auxiliary material for mRNA vaccine manufacturing and related applications.
 

Applications

mRNA vaccine production
 

Component

1. DNase I: 5 ml, 1 U/μl, GMP Grade
 

Storage

Store at -20°C