| Applications: | IF/IC, FC |
| Host Species: | Goat |
| Isotype: | IgG |
| Conjugation: | ABflo® 405. Ex:403nm. Em:427nm. |
| Immunogen: | Rabbit IgG |
| Dilution: | IF/IC 1:100-1:500; FC 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/ml |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 |
Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2.5μg/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 405-conjugated Goat Anti-Rabbit IgG (H+L) (AS056) at 1:100. |
 |
Confocal imaging of HeLa cells using β-Tubulin mAb(A12289, dilution 1:200) followed by a further incubation with ABflo® 405-conjugated Goat Anti-Rabbit IgG (H+L) (AS056, dilution 1:800)(Blue). Objective: 100x. |