| Applications: | WB, ELISA |
| Host Species: | Mouse |
| Isotype: | IgG1,Lambda |
| Conjugation: | HRP |
| Gene Name: | actin beta |
| Gene Symbol: | ACTB |
| Synonyms: | BRWS1; PS1TP5BP1; HRP-conjugated β-Actin |
| Gene ID: | 60 |
| UniProt ID: | P60709 |
| Clone ID: | 3W0O2 |
| Immunogen: | Recombinant protein of Mouse HRP-conjugated β-Actin |
| Dilution: | WB 1:2000-1:50000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.41 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C and avoid freeze/thaw cycles. |
| Documents: | Manual |
Images
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Western blot analysis of various lysates using HRP-conjugated β-Actin Mouse mAb (AC043) at 1:5000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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Western blot analysis of lysates from HeLa, using HRP-conjugated β-Actin Rabbit mAb (AC043) at 1:2000- 1:640000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |