tissue homogenates, cell lysates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
11783
UniProt ID:
O88879
Component:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables cysteine-type endopeptidase activator activity involved in apoptotic process and identical protein binding activity. Involved in intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress. Acts upstream of or within several processes, including nervous system development; positive regulation of cysteine-type endopeptidase activity; and regulation of apoptotic DNA fragmentation. Located in cytosol and nucleus. Is expressed in several structures, including inner cell mass; inner ear; limb interdigital region; liver; and vibrissa. Used to study Noonan syndrome. Orthologous to human APAF1 (apoptotic peptidase activating factor 1).
