tissue homogenates, cell lysates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
12846
UniProt ID:
O88587
Components:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables identical protein binding activity. Predicted to be involved in extracellular matrix organization. Located in basement membrane. Is expressed in several structures, including basement membrane; glomerular basement membrane of mature renal corpuscle; palatal shelf; pigmented retinal epithelium; and skin. Used to study recessive dystrophic epidermolysis bullosa. Human ortholog(s) of this gene implicated in epidermolysis bullosa dystrophica (multiple) and nonsyndromic congenital nail disorder 8. Orthologous to human COL7A1 (collagen type VII alpha 1 chain).
