tissue homogenates, cell lysates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
16362
UniProt ID:
P15314
Components:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables DNA-binding transcription factor activity, RNA polymerase II-specific and sequence-specific DNA binding activity. Involved in several processes, including defense response to symbiont; regulation of T cell activation; and regulation of gene expression. Acts upstream of or within CD8-positive, alpha-beta T cell differentiation; positive regulation of interleukin-12 production; and positive regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Is expressed in several structures, including bone marrow; early conceptus; eye; genitourinary system; and gut. Human ortholog(s) of this gene implicated in asthma; lung cancer (multiple); primary immunodeficiency disease; smallpox; and stomach cancer (multiple). Orthologous to human IRF1 (interferon regulatory factor 1).
