serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
53357
UniProt ID:
P97819
Component:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables calcium-independent phospholipase A2 activity; long-chain fatty acyl-CoA hydrolase activity; and serine hydrolase activity. Involved in positive regulation of insulin secretion involved in cellular response to glucose stimulus. Predicted to be located in several cellular components, including extracellular space; microtubule cytoskeleton; and nuclear speck. Predicted to be active in mitochondrion. Is expressed in 1st branchial arch maxillary component; olfactory placode; otic pit; and trunk somite. Used to study Parkinson's disease 14 and neurodegeneration with brain iron accumulation 2a. Human ortholog(s) of this gene implicated in dystonia 12; neuroaxonal dystrophy; and neurodegenerative disease (multiple). Orthologous to human PLA2G6 (phospholipase A2 group VI).
