1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Predicted to enable L-leucine binding activity and oxidoreductase activity, acting on peroxide as acceptor. Involved in cellular response to amino acid stimulus; negative regulation of TORC1 signaling; and negative regulation of cell growth. Acts upstream of or within regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction. Predicted to be located in cytoplasm; fibrillar center; and nucleoplasm. Predicted to be part of GATOR2 complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system; and integumental system. Orthologous to human SESN1 (sestrin 1).
