tissue homogenates, cell lysates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
72432
UniProt ID:
Q148R4
Components:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables peptidase inhibitor activity. Acts upstream of or within negative regulation of serine-type peptidase activity and regulation of cell adhesion. Predicted to be located in several cellular components, including endoplasmic reticulum membrane; epidermal lamellar body; and perinuclear region of cytoplasm. Predicted to be active in extracellular region. Is expressed in back skin. Used to study Netherton syndrome. Human ortholog(s) of this gene implicated in Netherton syndrome. Orthologous to human SPINK5 (serine peptidase inhibitor Kazal type 5).
