serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
50672
UniProt ID:
P21451
Components:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables several functions, including ATP binding activity; MAP kinase activity; and mitogen-activated protein kinase kinase kinase binding activity. Involved in several processes, including ERK1 and ERK2 cascade; cellular response to cAMP; and positive regulation of macromolecule biosynthetic process. Acts upstream of or within peptidyl-serine phosphorylation. Located in several cellular components, including caveola; dendrite cytoplasm; and perikaryon. Part of protein-containing complex. Is active in postsynaptic density. Used to study several diseases, including alcohol dependence; cocaine abuse; coronary artery disease (multiple); drug dependence (multiple); and gastric ulcer. Biomarker of alcohol use disorder; colon cancer; renal fibrosis; urethral obstruction; and vascular dementia. Human ortholog(s) of this gene implicated in several diseases, including Noonan syndrome 13; Parkinson's disease; adenocarcinoma (multiple); pulmonary hypertension; and renal cell carcinoma. Orthologous to human MAPK1 (mitogen-activated protein kinase 1).
