serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Experimental method:
Competitive inhibition
Shelf life:
12 months
Gene ID:
29141
UniProt ID:
P10683
Components:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables glutamate binding activity; glutamate decarboxylase activity; and pyridoxal phosphate binding activity. Involved in several processes, including carboxylic acid metabolic process; cellular response to brain-derived neurotrophic factor stimulus; and response to dexamethasone. Located in several cellular components, including inhibitory synapse; neuronal cell body; and synaptic vesicle membrane. Is active in GABA-ergic synapse and presynapse. Biomarker of autism spectrum disorder; bipolar disorder; epilepsy; transient cerebral ischemia; and withdrawal disorder. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; amphetamine abuse; diabetes mellitus (multiple); drug psychosis; and heroin dependence. Orthologous to human GAD2 (glutamate decarboxylase 2).
