1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables glutamate-cysteine ligase activity. Involved in several processes, including cellular response to follicle-stimulating hormone stimulus; cellular response to hepatocyte growth factor stimulus; and cellular response to mechanical stimulus. Part of glutamate-cysteine ligase complex. Used to study liver cirrhosis and pulmonary emphysema. Human ortholog(s) of this gene implicated in congenital nonspherocytic hemolytic anemia 7; cystic fibrosis; hemolytic anemia; and myocardial infarction. Orthologous to human GCLC (glutamate-cysteine ligase catalytic subunit).
