tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
29340
UniProt ID:
P09216
Components:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables several functions, including SH3 domain binding activity; diacylglycerol-dependent, calcium-independent serine/threonine kinase activity; and protein serine/threonine kinase binding activity. Involved in several processes, including cellular response to platelet-derived growth factor stimulus; negative regulation of mitochondrial calcium ion concentration; and negative regulation of mitochondrial membrane potential. Located in Golgi membrane and T-tubule. Is active in glutamatergic synapse; neuromuscular junction; and presynaptic cytosol. Used to study hypertension. Biomarker of type 2 diabetes mellitus. Orthologous to human PRKCE (protein kinase C epsilon).
