tissue homogenates, cell lysates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
81516
UniProt ID:
O88406
Components:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables ubiquitin protein ligase binding activity. Involved in intracellular signal transduction; negative regulation of DNA-templated transcription; and regulation of transforming growth factor beta receptor signaling pathway. Predicted to be located in several cellular components, including adherens junction; centrosome; and nuclear lumen. Predicted to be part of heteromeric SMAD protein complex. Predicted to be active in plasma membrane. Biomarker of nephritis and pulmonary hypertension. Human ortholog(s) of this gene implicated in colorectal cancer and pancreatic adenocarcinoma. Orthologous to human SMAD7 (SMAD family member 7).
