| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Gene Name: | glyceraldehyde-3-phosphate dehydrogenase |
| Gene Symbol: | GAPDH |
| Synonyms: | G3PD; GAPD; HEL-S-162eP; GAPDH (Human Specific) |
| Gene ID: | 2597 |
| UniProt ID: | P04406 |
| Clone ID: | 1D3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2). |
| Dilution: | WB 1:50000-1:200000 IF/IC 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
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Western blot analysis of GAPDH expression in (1) HeLa cell lysate; (2) MDBK cell lysate; (3) COS-1 cell lysate; (4) MDCK cell lysate with GAPDH Antibody. |
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Western blot analysis of various lysates using GAPDH (Human Specific) Rabbit mAb (AC036) at 1:100000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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Immunofluorescence analysis of U-2 OS cells using GAPDH (Human Specific) Rabbit mAb (AC036) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |