Fluorescent Mounting Medium with DAPI (25 ml)

Fluorescent Mounting Medium with DAPI (25 ml)

Catalog #: C0004
Availability: In Stock
¥199.00

荧光封片剂是专门配制的,用于延长免疫细胞化学和免疫组织化学染色中荧光的强度。如果将玻片储存在-20C,我们的产品可以将荧光染料的漂白时间延长至少两个月。

产品特色

• 即使在第12次激光扫描后,仍能保持出色的信号。

• 在-20°C下可抑制光漂白至少4个月。

• 即开即用,简单易用。

• 可提供带或不带DAPI复染。

产品组分

1. 含DAPI的荧光封片剂:25毫升。

存储条件

储存于4°C并避光保存。

案例

左图:Hela细胞用CTCF抗体染色,并使用含有DAPI的FadeStop™荧光封片剂进行封片。载玻片-20°C下储存1个月后,使用蔡司LSM 880共聚焦激光扫描显微镜进行成像。进行了一系列扫描以评估光染料的光漂白速度。值得注意的是,FadeStop™荧光封片剂有效地保护了荧光。即使在第12次扫描后,图像依然保持良好。

右图:使用CTCF抗体对Hela细胞进行染色,并将其封存于含有DAPI的FadeStop™荧光封片剂中。免疫光染色后1天、2个月和4个月,使用Zeiss LSM 880共聚焦激光扫描显微镜进行图像采集。细胞核用DAPI成蓝色。ACTB用Alexa Fluor 647染成洋红色。比例尺:20 μm。

Publications

1. Preserving Endothelial Integrity in Human Saphenous Veins during Preparation for Coronary Bypass Surgery.
Publication: JVR        Product: FadeStop™ fluorescent mounting reagent with DAPI
 

Customer Testimonials

"I used anti-fade fluorescent mounting medium in immunocytochemistry and immunohistochemistry for over 7 years. Compared to other brands, FadeStop™ Mounting Medium stands out so well. It not only preserved the fluorescence of my samples when I used laser-scanning confocal microscopy, but it also gave me convenience by prolonging the fluorescence after staining. So now I can take my time by storing my slides in -20°C for a long period of time without having to take images immediately after staining."

------ Virginia Tech, Dr. Larry Eaton, Postdoc
 

""I tried FadeStop Fluorescent™ Mounting Medium with DAPI on fixed samples of Hydra vulgaris. The DAPI staining was bright and fast and the mounting medium effectively preserved fluorescent phalloidin stain. Thank you for an excellent product!""

------ University of San Diego, Dr. Callen Hyland, Lab manager