LiDirect™ MagBead DNA Methylation Kit (96 preps)

LiDirect™ MagBead DNA Methylation Kit combines DNA denaturation and bisulfite conversion into a single step, coupled with magnetic bead–based cleanup to enable high-throughput methylation analysis. The kit is streamlined to achieve high-yield DNA recovery following bisulfite conversion. Desulfonation and cleanup of the converted DNA are performed while the DNA is bound to MagPure Beads. The kit is optimized to minimize template degradation and DNA loss during treatment and cleanup, while ensuring complete conversion of unmethylated cytosines. The recovered DNA is ideal for PCR amplification and downstream applications, including endonuclease digestion, sequencing, microarrays, and more.
 

Key Features

• Complete, high-throughput bisulfite conversion of DNA in less than 2 hours.

• A coupled heat denaturation and conversion step streamlines the conversion of unmethylated cytosines to uracil.

• High-throughput (96-well), automated desulfonation and recovery of bisulfite-treated DNA.

• Recovered DNA is suitable for downstream analyses such as PCR, endonuclease digestion, sequencing, and microarrays.
 

Specifications

1. Conversion: >99%
2. Elution Volume: ≥25 μl
3. Input: 500 pg – 2 μg of DNAA
4. Process Time: 2 hours
5. Recovery: >70%
 

Applications

Library preparation for next generation sequencing, PCR amplification, etc.
 

Components

1. CT Conversion Reagent: 1 bottle
2. Dilution Buffer: 3.5 ml
3. Dissolving Buffer: 600 µl
4. Binding Buffer: 60 mL
5. Wash Buffer: 15 ml
6. Desulphonation Buffer: 20 ml
7. Elution Buffer: 2x 1.5 ml
8. Magnetic bead: 2 ml
 

Storage

Store at room temperature