2× LiTaq™ PCR 预混液是一种优化的即用型溶液，含有Taq DNA聚合酶、dNTPs、MgCl2、KCl和稳定剂。2× Taq Master Mix在-20°C下储存时，可稳定承受20次冻融循环。两种追踪染料的存在使得PCR产物可以直接加样到琼脂糖凝胶中。获得的PCR产物与LiClone™ 一步克隆试剂盒 (M0010)兼容，并且可以直接用于克隆到T-Vectors中，因为大多数用Taq DNA聚合酶扩增的PCR产物在3'-末端都有一个A。
产品特色
• PCR产物可以直接用于克隆到T载体中，因为大多数用Taq DNA聚合酶扩增的PCR产物在3'末端都有一个A。
• 在-20°C下储存时，可稳定经受20次冻融循环。
• 成本极低，非常适合基因分型。
产品应用
常规PCR
产品组分
1. 2× LiTaq™ PCR 预混液 (+Dye): 15×1 ml
存储条件
-20°C保存
其它规格
| 产品名称 | 货号 | 价格 | |
| 2× LiTaq™ PCR Master Mix (+Dye) (5× 1ml) | M0024-05 | ￥200.00 |  |
| 2× LiTaq™ PCR Master Mix (+Dye) (50× 1ml) | M0024-50 | ￥1650.00 | |
| 2× LiTaq™ PCR Master Mix (-Dye) (15× 1ml) | M0024-03 | ￥550.00 | |
| 2× LiTaq™ PCR Master Mix (-Dye) (50× 1ml) | M0024-04 | ￥1650.00 | |
成功案例
样品: 人基因组DNA & cDNA, 小鼠基因组DNA, 小麦基因组DNA, 大鼠基因组DNA, 大肠杆菌菌落
LIFESCT: LiTaq™ PCR 预混液, LiTaq™ DNA聚合酶, 2× LiTaq™ plus PCR 预混液, 2× LiTaq™ Eco PCR 预混液
竞品: Brand C, Brand N, Brand Q, Brand T, Brand G, Brand F
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用于扩增的 PCR 反应体系： |
用于扩增的PCR循环体系： |
从上述结果可以得出结论，对于不同长度和类型的模板进行PCR扩增，我们公司的所有四种PCR混合物均表现。对于常规PCR，我们的产品具有最广泛的适用性、最高的成功率、最佳的特异性和最大的产量。在最困难的情况下，当所有PCR混合物都失败时，我们至少有一款产品仍然有效。
发表论文
1. Nuclear export and translation of circular repeat-containing intronic RNA in C9ORF72-ALS/FTD.
Publication: Nature Communications Product: 2× LiTaq™ PCR Master Mix (+Dye)
2. Distinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms.
Publication: Molecular Cell Product: 2× LiTaq™ PCR Master Mix (+Dye)
3. In Vitro Analysis of Rationally Designed Multimerization Sequences.
Publication: ProQuest Product: LiTaq™ Plus PCR Master Mix (+Dye)
客户评价
"Our lab started to use the high-quality services from LifeSct, including the gene synthesis and other molecular biology services since 2017. We are always happy with the great quality of the services, the reasonable price, the prompt shipment and the good communication from LifeSct. More impressively, your LiTaq™ PCR Master Mix is robust and cost-effective. Thanks for continuing to produce great products and provide excellent services!"
"I'm citing 2× LiTaq™ Plus PCR Master Mix (LifeSct LLC) in my manuscript for a protein engineering paper. It is extremely effective, and I really appreciated the sample. I'll send you a copy as soon as it's published.BTW, LiScript™ Fast RT Master Mix works great, actually I wanted to mention how fast and easy it was to use. The addition of RNAse inhibitor and the 5x concentration are perfect for fast experiments."
"I have been using LiTaq™ Plus PCR Master Mix and LiQuant™ Universal Green qPCR master mix in my experiments for two years. The PCR mix can impressively amplify the difficult sequences in my hands. Amazingly, it rapidly amplifies the templates at ~ 2kb/min. And the qPCR mix has the higher sensitivity compared with the similar product from other company. I highly recommend these products!!!"
"Our Lab had a chance to try the LiTaq™ Plus PCR Master Mix and LiClone™ One Step DNA Assembly Kit which perform very well compared with similar products we used before. We will order them later. Thank you!"