2× LiTaq™ Plus PCR预混液是一种由 LiTaq™ DNA 聚合酶、dNTP、优化的缓冲液系统以及含有 3'→5' 外切酶活性的酶组成的混合物。其保真度是 Taq DNA 聚合酶的 6 倍。与 Taq DNA 聚合酶相比，LiTaq™ Plus DNA 聚合酶具有更强的扩增性能、更高的灵敏度，并且在 5 kb 扩增范围内对杂质的耐受性更强。它能从人类基因组 DNA 中扩增高达 10 kb，或从 λDNA 中扩增高达 15 kb。2× Taq Plus Master Mix 中的保护剂使其能够抵抗反复的冻融循环。获得的 PCR 产物与 LiClone™ One Step Cloning Kit（目录号：M0010）兼容，并且可以直接用于克隆到 T-Vectors 中，因为大多数用 Taq DNA 聚合酶扩增的 PCR 产物在 3' 端都有一个 A。
产品特色
• PCR产物可以直接用于克隆到T载体中，因为大多数用Taq DNA聚合酶扩增的PCR产物在3'末端都有一个A。
• 在-20°C下储存时，可稳定经受20次冻融循环。
• 成本极低，非常适合基因分型。
产品应用
1. 常规PCR
2. 基因分析
产品组分
1. 2× LiTaq™ Plus PCR预混液 (-Dye): 50× 1ml
存储条件
-20°C保存
其它规格
| 产品名称 | 货号 | 价格 | |
| 2× LiTaq™ Plus PCR预混液 (+Dye) (5× 1ml) | M0025-05 | ￥290.00 |  |
| 2× LiTaq™ Plus PCR预混液 (+Dye) (15× 1ml) | M0025-15 | ￥790.00 | |
| 2× LiTaq™ Plus PCR预混液 (+Dye) (50× 1ml) | M0025-50 | ￥2490.00 | |
| 2× LiTaq™ Plus PCR预混液 (-Dye) (15× 1ml) | M0025-03 | ￥790.00 | |
成功案例
样品: 人基因组DNA & cDNA, 小鼠基因组DNA, 小麦基因组DNA, 大鼠基因组DNA, 大肠杆菌菌落
LIFESCT: LiTaq™ PCR 预混液, LiTaq™ DNA聚合酶, 2× LiTaq™ plus PCR 预混液, 2× LiTaq™ Eco PCR 预混液
竞品: Brand C, Brand N, Brand Q, Brand T, Brand G, Brand F
|
用于扩增的 PCR 反应体系： |
用于扩增的PCR循环体系： |
从上述结果可以得出结论，对于不同长度和类型的模板进行PCR扩增，我们公司的所有四种PCR混合物均表现。对于常规PCR，我们的产品具有最广泛的适用性、最高的成功率、最佳的特异性和最大的产量。在最困难的情况下，当所有PCR混合物都失败时，我们至少有一款产品仍然有效。
发表论文
1. Nuclear export and translation of circular repeat-containing intronic RNA in C9ORF72-ALS/FTD.
Publication: Nature Communications Product: 2× LiTaq™ PCR Master Mix (+Dye)
2. Distinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms.
Publication: Molecular Cell Product: 2× LiTaq™ PCR Master Mix (+Dye)
3. In Vitro Analysis of Rationally Designed Multimerization Sequences.
Publication: ProQuest Product: LiTaq™ Plus PCR Master Mix (+Dye)
客户评价
"Our lab started to use the high-quality services from LifeSct, including the gene synthesis and other molecular biology services since 2017. We are always happy with the great quality of the services, the reasonable price, the prompt shipment and the good communication from LifeSct. More impressively, your LiTaq™ PCR Master Mix is robust and cost-effective. Thanks for continuing to produce great products and provide excellent services!"
"I'm citing 2× LiTaq™ Plus PCR Master Mix (LifeSct LLC) in my manuscript for a protein engineering paper. It is extremely effective, and I really appreciated the sample. I'll send you a copy as soon as it's published.BTW, LiScript™ Fast RT Master Mix works great, actually I wanted to mention how fast and easy it was to use. The addition of RNAse inhibitor and the 5x concentration are perfect for fast experiments."
"I have been using LiTaq™ Plus PCR Master Mix and LiQuant™ Universal Green qPCR master mix in my experiments for two years. The PCR mix can impressively amplify the difficult sequences in my hands. Amazingly, it rapidly amplifies the templates at ~ 2kb/min. And the qPCR mix has the higher sensitivity compared with the similar product from other company. I highly recommend these products!!!"
"Our Lab had a chance to try the LiTaq™ Plus PCR Master Mix and LiClone™ One Step DNA Assembly Kit which perform very well compared with similar products we used before. We will order them later. Thank you!"