LiPure™ Tissue DNA Extraction Kit provides a rapid and easy method for isolating DNA from fresh or frozen animal tissue samples. The DNA purification process is simplified using spin column technology into four quick steps—lyse, bind, wash, and elute—and can be completed in less than 20 minutes post-lysis. This convenient spin-column format eliminates time-consuming steps such as alcohol precipitation and the use of toxic compounds like phenol and chloroform, while also allowing multiple samples to be processed in parallel. DNA purified with this kit is ready for most downstream applications, including PCR, sequencing, genotyping, Southern blot analysis, and restriction enzyme digestion.
Key Features
• Fast: DNA purification in less than 20 minutes post-lysis.
• Safe: No phenol/chloroform extraction required.
• High-quality: Purified DNA is suitable for a variety of downstream applications.
Specifications
1. Sample Source: Fresh and frozen tissues, paraffin-embedded tissues, cells, mouse tail snips, blood, and buccal swabs.
2. Sample Input: 10-25 mg tissues, 5×10^6 cultured cells
3. Elution Volume: 30-100 μl
4. Technology: Silica spin column
Applications
Total DNA isolation from fresh and frozen animal tissues.
Components
1. Buffer ATL: 15 ml
2. Buffer DL: 15 ml
3. Buffer AW1: 15 ml
4. Buffer AW2: 15 ml
5. Buffer AE: 15 ml
6. Proteinase K: 1 ml, 20 mg/ml
7. Mini Column: 50×
8. Collection Tub: 50× 2ml
Storage
Store at -20°C for proteinase K; Room temperature for other.
Case Study
Genomic DNA from different tissue samples was isolated using LiPure™ Tissue DNA Extraction Kit. Eluted DNA was analyzed on a 1% agarose gel.
Genomic DNA yield and quality using LiPure™ Tissue DNA Extraction Kit.