LiQuant™ Universal Green qPCR Master Mix is a premium, ready-to-use 2× premix designed for real-time quantitative PCR (qPCR) utilizing the SYBR Green I chimeric fluorescence method.
At the core of this formulation is LiQuant™ Taq Pro DNA Polymerase, a next-generation hot-start polymerase modified via an advanced antibody method. Engineered for excellence, it delivers exceptional specificity, ultra-high detection sensitivity, and maximum amplification yields.
By combining an optimized qPCR buffer system with proprietary specificity enhancers, this master mix ensures robust performance even with challenging templates. It simplifies your workflow by requiring only the addition of primers and your DNA template.
Key Features
• Stable Low-Expression Gene Detection: Features a novel antibody-modified hot-start Taq Pro DNA Polymerase. Equipped with a specificity-enhancing factor, it delivers the high amplification performance required to detect low-copy-number targets reliably.
• Universal ROX Passive Reference Dye: Compatible with all qPCR instruments. Eliminates the need to adjust ROX dye concentrations for different thermal cyclers, saving time and reducing pipetting errors.
• Superior Fluorescence Intensity: Significantly boosted fluorescence output generates clean, robust, and graceful amplification curves for reliable data analysis.
Applications
1. Gene expression analysis
2. Low-copy gene detection
3. High-throughput qPCR screening
4. Validation of RNAi and microarray data
Component
1. LiQuant™ Universal Green Master Mix, V2.0: 4× 1.25ml
Storage
Store at -20°C and protected from light.
Other Size
| Product Name | Cat. # | Price | View |
| LiQuant™ Universal Green qPCR Master Mix, V2.0 (2500 rxns) | M0016-25N | $1459 |  |
Case Study
Figure A illustrates an 8-point, 10-fold serial dilution of the pUC19 plasmid, while Figure B displays the corresponding standard curve generated from the cycle threshold (CT)) values in Figure A. Notably, the lowest plasmid concentration (the eighth dilution gradient) contains approximately 6 copies per 4 µL reaction. Each dilution point was assayed using the LiQuant™ Universal Green qPCR Master Mix with a template volume of 4 µL per well. The results demonstrate that LiQuant™ exhibits an excellent linear relationship across a broad dynamic range, delivering high-sensitivity detection capable of quantifying single-digit template copy numbers.
In Figure C, cDNA derived from HeLa cells served as the template to evaluate six distinct genes under identical amplification conditions. This assay compared the performance of LiQuant™ against SYBR qPCR reagents from three other commercial suppliers. The results show that LiQuant™ successfully detected 92% (22/24) of the target genes with exceptional specificity.
Publications
1. YAP/TEAD1 Complex Is a Default Repressor of Cardiac Toll-Like Receptor Genes.
Publication: International Journal of Molecular Sciences Product: LiQuant™ Universal Green qPCR Master Mix
2. Knockdown of lncRNA TP53TG1 Enhances the Efficacy of Sorafenib in Human Hepatocellular Carcinoma Cells.
Publication: non-coding RNA Product: LiQuant™ Universal Green qPCR Master Mix
3. Selectively expressing SARS-CoV-2 Spike protein S1 subunit in cardiomyocytes induces cardiac hypertrophy in mice.
Publication: bioRxiv Product: LiQuant™ Universal Green qPCR Master Mix