LiPure™ Universal RNA Extraction Kit delivers rapid, high-yield extraction of total RNA from a broad spectrum of sample sources, including animal tissues, cultured cells, plant and fungal tissues, bacteria, and whole blood.
The system utilizes a specially formulated lysis buffer to rapidly disrupt cells while selectively precipitating cellular proteins and genomic DNA. The RNA-rich supernatant is then transferred directly to a silica spin column for efficient washing and impurity removal, concluding with a fast elution in DEPC-treated water. This streamlined workflow takes less than 30 minutes and yields intact, high-purity RNA ready for demanding downstream applications—including RT-PCR, RT-qPCR, Northern blotting, next generation sequencing (NGS), mRNA isolation, nuclease protection assays, and in vitro translation.
Key Features
• Fast: Complete RNA isolation workflow in less than 30 minutes.
• Versatile: One optimized kit for animal, plant, fungal, bacterial, and blood samples.
• High-Quality: Consistently yields pure, intact RNA optimized for sensitive downstream assays.
Specifications
1. Sample Source: Animal tissues and cells, plant and fungal tissues, bacteria, and blood
2. Elution Volume: 50-100 μl
3. Technology: Silica spin column
Applications
Genomic RNA from a variety of sample sources including animal tissues and cells, plant and fungal tissues, bacteria, and blood.
Components
1. Buffer RL: 55 ml
2. Buffer AW2: 10 ml
3. DEPC Wate: 10 ml
4. Mini Column: 50×
5. Collection Tube: 50× 2ml
Storage
Store at room temperature
Case Study
RNA from different tissue samples was isolated using LiPure™ Universal RNA Extraction Kit. Eluted DNA was analyzed on a 1% agarose gel.