Custom Stable Cell Lines
Stable cell lines are essential for functional genomics and animal disease modeling because they eliminate the variability and transient expression of standard transfection. LIFESCT provides a robust platform for engineering both overexpression and knockdown stable cell lines. Using high-titer lentiviral transduction and stringent antibiotic selection, we deliver high integration efficiency and consistent transgene expression across diverse mammalian cell types.
Key Features
• LIFESCT offers a one-stop solution covering primer synthesis, gene synthesis, viral packaging, and cell engineering, enabling fast and efficient project delivery.
• Our stable overexpression cell lines are generated using lentiviral transduction combined with antibiotic selection, enabling the establishment of stable cell lines across a wide range of cell types.
Service Options
| Service Type | Description | Deliverables | Lead Time |
| Overexpression Cell Lines | • Gene construction & lentivirus packaging (gene≤3kbp) • qPCR and western blot validation |
Target, control ,and wild-type cells, >10^6 cells/tube *2 tubes for each type of cell | 70-80 business days |
| Knockdown RNAi Cell Lines | • Gene construction & lentivirus packaging • qPCR and western blot validation |
Target, control ,and wild-type cells, >10^6 cells/tube *3 tubes for each type of cell | 70-80 business days |
Quote Guide
To inquire about this service, please choose one of the options below:
1. Fill out Enquiry Form to submit your request.
2. Download the Quote Form and send to sales@lifesct.com.
3. Register and log in to your account to submit your request via Service Quote system.
Case Study
Fluorescence imaging and Western blot analysis demonstrating stable overexpression of the target gene in KYSE-150 cells.