LiScript™ First-Strand cDNA Synthesis Kit contains all the components necessary for the 1st strand cDNA synthesis. The products are suitable for PCR and qPCR. The 2× RT Mix contains an optimized buffer and dNTPs. The Enzyme Mix contains the Reverse Transcriptase and the RNase inhibitor. The Oligo-(dT)23VN has a better affinity to Ploy A+ RNA than Oligo (dT)18. In addition, random hexamers and gene-specific primers (GSP) are also optional.
 

Key Features

• Based on high-effective Reverse Transcriptase: with high thermal stability ensuring the complex RNA secondary structure be denatured to obtain longer cDNA.

• A wide range of template input amount of total RNA (from 1 pg to 5 μg). Amplify cDNA fragments as long as 20 kb.

• Anchored Oligo(dT)23VN: further improves the specificity, efficiency and sensitivity of the first-strand cDNA synthesis.

• With different primer selection strategy, the first-strand cDNA products can be widely used for many applications, including cloning, hybridization, PCR amplification, and Real Time PCR.
 

Product Name	Cat. #	Price	View
LiScript™ First-Strand cDNA Synthesis Kit (25 rxns)	M0028-01	$119
LiScript™ One Step RT-PCR Kit (100 rxns)	M0017	$189
LiScript™ Fast RT Master Mix (100 rxns)	M0029	$269
LiScript™ Reverse Transcriptase (5000 U)	M0022	$139
LiScript™ SYBR Green One-Step RT-qPCR Kit (200 rxns)	M0032	$299
LiScript™ Probe One-Step RT-qPCR Kit (100 rxns)	M0033	$199

Case Study

cDNA fragments as long as 20 kb was successfully obtained from RNA templates using LiScript™ 1st Strand cDNA Synthesis Kit (Left Figure). Reverse transcription was performed using 1 μg total RNA of HeLa cells or total RNA (for Nebulin) of skeletal muscle cells as templates and Oligo(dT)23VN as primers. PCR amplification was performed using 1 μl of cDNA (as template) and DNA Polymerase.

LiScript™ has higher reverse transcription efficiency for complex RNA templates (Right Figure). Using 1 μg of total RNA of Hela cells as template, reverse transcription was performed using LiScript™ and a similar product of T brand, respectively. The reaction time is 5 min. Using 1 μl of cDNA as template, the high-GC regions of FIB (6100 bp) and Pole (7117bp) were amplified using LiQuant™ Green qPCR Master Mix, respectively.

LiScript™ has a wide and sensitive linear reverse transcription range for qPCR. Reverse transcription was perform using 1 μg-1 pg total RNA of Hela cells as template and random hexamers/Oligo(dT)23VN as mixed primers. qPCR amplification of amplify ACTB gene and BR3 gene was performed using 1 μl of cDNA (as template) and LiQuant™ Green qPCR Master Mix, respectively.