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  • LiTaq™ PCR Master Mixes

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LiTaq™ PCR Master Mixes

LiTaq™ PCR Master Mixes

LiTaq™ DNA Polymerase is a thermostable DNA polymerase that exhibits a 5'→3 ' polymerase activity and a 5'→3 ' exonuclease activity, with no 3' →5 ' exonuclease activity. LiTaq™ DNA Polymerase is purified from an Escherichia coli (E.coli) strain overexpressing the gene of Thermus aquaticus DNA Polymerase. No endonuclease, exonuclease, or bacterial DNA were detected in this enzyme.
 

Selection Guide


 

2× LiTaq™ PCR Master Mix (M0024) is an optimized ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2, KCI and stabilizers; 2× LiTaq™ Plus PCR Master Mix (M0025) is a mixture of LiTaq™ plus DNA polymerase, dNTP, an optimized buffer system and an enzyme containing 3’→5’ exonuclease activity. Its fidelity is 6 times greater than that of 2× LiTaq™ Master Mix. LiTaq™ PCR mix and LiTaq™ Plus PCR mix are very cost-effective and ideal for genotyping.

LiTaq™ Ultra HiFi PCR Master Mix (M0031) is a ready-to-use super-fidelity PCR master mix designed for superior accuracy and performance. This high fidelity PCR mix incorporates a genetically engineered HotStart high fidelity DNA polymerase, delivering exceptional DNA affinity and processivity. It excels with complex and partially degraded templates, offering a fidelity >100× higher than conventional taq polymerase. 

 

Product Name Cat. # Price View
2× LiTaq™ PCR Master Mix (+Dye) (5× 1ml) M0024-05 $99
2× LiTaq™ PCR Master Mix (-Dye) (15× 1ml) M0024-03 $229
2× LiTaq™ Plus PCR Master Mix (+Dye) (5× 1ml) M0025-05 $139
2× LiTaq™ Plus PCR Master Mix (-Dye) (15× 1ml) M0025-03 $349
LiTaq™ DNA Polymerase (10000 U) M0023 $599
2× LiTaq™ HotStart PCR Master Mix (+Dye) (5× 1ml) M0021 $159
2× LiTaq™ HotStart HiFi PCR Master Mix (+Dye) (5× 1ml) M0006 $219
2× LiTaq™ Ultra HiFi PCR Master Mix (1 ml) M0031 $129
LiTaq™ Ultra HiFi DNA Polymerase (500 U) M0030 $399


Case Study

Samples: Human genomic DNA & cDNA, Mouse genomic DNA, Wheat genomic DNA, Rice genomic DNA, Coli colony

LIFESCT: LiTaq™ PCR mix, LiTaq™ DNA polymerase, 2× LiTaq™ plus PCR mix

Competitors: Brand C, Brand N, Brand Q, Brand T, Brand G, Brand F

PCR reaction setup for amplification:
• 2× Mix: 12.5 µl
• F1: 1 µl
• R1 : 1 µl
• Template* : 0.5 µl
• ddH2O: 0.2 ul
* gDNA: 50 ng; λ DNA: 5 ng; Plasmid: 2 ng;  Colony: 1 µl

PCR cycling for amplification:
1. 95°C: 3 min
2. 35 x
    1. Denaturation: 15 s at 95°C
    2. Annealing: 20 s at 60°C
    3. Extension: 1 min/kb at 72°C
3. Final extension: 5 min at 72°C

From the above results, it can be concluded that for PCR amplification of different length and kind of templates, all four PCR mixes from our company performed superbly. For general PCR, our products have the broadest applicability, best success rate, highest specificity and maximal yield. In the most difficult situations, when all other PCR mixes failed, at least one of our products still worked.
 

Publications

1. Nuclear export and translation of circular repeat-containing intronic RNA in C9ORF72-ALS/FTD. 
Publication: Nature Communications        Product: 2× LiTaq™ PCR Master Mix (+Dye)

2. Distinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms. 
Publication: Molecular Cell        Product: 2× LiTaq™ PCR Master Mix (+Dye)

3. In Vitro Analysis of Rationally Designed Multimerization Sequences. 
Publication: ProQuest        Product: 2× LiTaq™ Plus PCR Master Mix (+Dye)

 

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