Mammalian Cell Protein Expression
The Mammalian Cell Expression System is an ideal system for functional protein expression. Biological activity of recombinant proteins produced by this system closely resembles that of natural proteins. Because of its unique advantages, mammalian cell expression systems are widely used in the biopharmaceutical industry.
With our experienced scientific team, we has developed our own mammalian cell expression technology platform, capable of delivering recombinant proteins from the milligram level to the gram level. Combined with our advanced protein purification facilities, we can deliver recombinant protein products with purity 95% and above. Protein expression levels and success rates can be significantly improved using our proprietary Codon Optimization software.
Key Features
• Proprietary Codon Optimization Technology: significantly improve protein expression level and success rate.
• Proprietary signal peptide library: customize signal peptide according to the sequence of target protein.
• Multiple purification strategies: affinity chromatography, ion exchange, hydrophobic chromatography, gel filtration, etc., providing diversified choice of protein purities.
• Comprehensive protein identification services: SDS-PAGE, Western Blot, MALDI-TOF, HPLC, etc.
Specifications
| Process | Description | Delivery Form | EDT |
| 1. Gene synthesis | Codon Optimization & Gene synthesis | Plasmid DNA | 1-2 weeks |
| 2. Subcloning | Subcloning the target gene into expression vector | Constructs | 1 week |
| 3. Pilot protein expression and purification | Pilot protein expression and purification Test the expression level | Expression report | 1-2 weeks |
| 4. Large scale protein expression and purification | Scale-up expression and purification | Recombinant protein QC report | 1-2 weeks |
Quote Guide
To inquire about this service, please choose one of the options below:
1. Fill out Enquiry Form to submit your request.
2. Download the Quote Form and send to sales@lifesct.com.
3. Register and log in to your account to submit your request via Service Quote system.
