As a gene synthesis company, LIFESCT has provided customers with a large number of genes and plasmids to life scientists. By using our proprietary LiClone™ DNA synthesis and assembly platforms, the only thing you need to do is to tell us the DNA or amino acid sequences you are interested in. According to the DNA or amino acid sequences you provided, we will de novo synthesize the error-free DNA sequences and clone them into any vector as requested. In order to provide our customers with the highest quality service, Sanger sequencing will be conducted before each of these steps in order to verify the sequence accuracy. With our professional research team and effective systems in place, we promise you a one-stop location that will satisfy all of your gene synthesis needs.

Key Features

• Codon optimization software that provides free codon optimization services, enabling a significant increase in protein expression.

• LiClone™ Assembly technology ensures reliable assembly of long DNA fragments, single gene sequence up to 200 kb.

• Guarantee 100% sequence accuracy for all genes.
 

Service Options (Cat. #: S0012)

Custom Gene Synthesis

* For complex genes, need to quote;
** Free for subcloning to usual vectors (download the list);
** $49 for subcloning to unusual vectors;
*** Turnaround time is dependent on length and complexity of the genes ordered. In a few cases, shipping time may exceed the estimated time.

Rush Gene Synthesis

* Not applicable to genes with complex sequences.
 

Quote Guide

To inquire about this service, please choose one of the options below:
1. Fill out Enquiry Form to submit your request.
2. Download the Quote Form and send to sales@lifesct.com.
3. Register and log in to your account to submit your request via Service Quote system.
 

Delivery Forms

1. 1 tube of 2-5 μg lyophilized plasmid DNA
2. Original peak diagram of sequencing results (.ab1 format)
3. COA file, including QC enzyme digestion verification figure
 

Publications

1. Rapid 40S scanning and its regulation by mRNA structure during eukaryotic translation initiation.  
Publication: Cell 

2. HLA class I signal peptide polymorphism determines the level of CD94/NKG2–HLA-E-mediated regulation of effector cell responses.  
Publication: Nature Immunology

3. eEF2 diphthamide modification restrains spurious frameshifting to maintain translational fidelity.  
Publication: Nucleic Acids Research

4. The DNA Methyltransferase Inhibitor 5-Aza-4'-thio-2'-Deoxycytidine Induces C>G Transversions and Acute Lymphoid Leukemia Development. 
Publication: Cancer Research