ECL WB Detection Kits
LIFESCT offers two types of super sensitive chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP). The signal from ECL substrates can be captured and documented using X-ray film or a capable CCD camera imager.
Selection Guide
| Product | Sensitivity | Primary Ab Dilution | Secondary Ab Dilution |
| SuperECL™ | Picogram to low femtogram | 1:1,000-1:30,000 | 1:50,000-1:200,000 |
| SuperECL™ Plus | High Femtogram | 1:5,000-1:30,000 | 1:100,000-1:300,000 |
Key Features
• High sensitivity: detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane.
• High signal stability: incubated blots provide stable signal duration over 4 hour time period.
• Stable reagent: reagent is stable for one year.
• Cost-effective: use much less antibody.
| Product Name | Cat. # | Price | View |
| SuperECL™ Western Blotting Detection Kit (2×50 ml) | M0013-05 | $149 |  |
| SuperECL™ Western Blotting Detection Kit (2×250 ml) | M0013-25 | $349 | |
| SuperECL™ Plus Western Blotting Detection Kit (2×50 ml) | M0053-05 | $249 | |
| SuperECL™ Plus Western Blotting Detection Kit (2×100 ml) | M0053-10 | $389 | |
Case Study
The comparison of SuperECL™ plus and SuperSignal™ (Thermo) on exposure time.
The comparison on exposure time(Left); The comparison on signal duration (Right).
The comparison of sensitivity of SuperECL™, SuperECL™ Plus, and Immobilon™ (Millipore) Western blot substrate (Left); The comparison on antibody dilution (Right).