CCK-8, MTT Cell Proliferation Assay Kit and AlamarBlue Cell Viability Reagent utilize metabolic indicators to measure cellular reduction potential. In the CCK-8 assay, the WST-8 reagent is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue culture media. The amount of the formazan dye, generated by the activities of dehydrogenases in cells, is directly proportional to the number of living cells. In the MTT assay, the MTT reagent is reduced by metabolically active cells to a strongly colored reporter, formazan. The signal is proportional to the number of live cells, thus allowing an accurate quantification of changes in the rate of cell proliferation. Similarly, our AlamarBlue reagent (resazurin) is converted to the fluorescent and colorimetric reporter molecule resorufin in metabolically active cells.
 

The assay principle of CCK-8 and AlarmarBlue.
 

Product Name	Cat. #	Price	View
Cell Counting Kit-8 (500 rxns)	C0024	$139
MTT Cell Proliferation Assay Kit (1000 rxns)	C0025	$199
AlamarBlue Cell Viability Reagent (2500 rxns)	C0026	$179

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