N1-Methylpseudo-UTP (N1-Methyl-Pseudouridine-5'-Triphosphate) is a modified nucleoside triphosphate designed for incorporation into messenger RNAs (mRNAs) using T7 RNA polymerase. The inclusion of N1-methylpseudouridine reduces the immunogenicity of the resulting mRNA.
A recent study reported that the N1-methylpseudouridine (m1Ψ) modification, either alone or in combination with 5-methylcytidine (m5C), outperformed the current state-of-the-art pseudouridine (Ψ) or m5C/Ψ-modified mRNA platforms. Specifically, it achieved up to ~44-fold higher reporter gene expression in cells (with double-modified mRNAs) and ~13-fold higher expression in mice (with single-modified mRNAs). Furthermore, compared to (m5C/)Ψ-modified mRNAs, those modified with (m5C/)m1Ψ exhibited reduced intracellular innate immunogenicity and improved cellular viability following in vitro transfection. These findings suggest that N1-methylpseudo-UTP may serve as a valuable component for synthesizing druggable mRNAs with enhanced performance.
Specifications
1. CAS Number: 1428903-59-6 (free acid)
2. Concentration: 100 mM in H2O
3. Molecular Formula: C10H17N2O15P3 (free acid)
4. Molecular Weight: 497.98 (free acid)
5. Purity: >95% (HPLC)
6. Extinction Coefficient: 8,880 Lmol-1cm-1 at 271 nm
7. Salt Form: Na+
Component
1. N1-Methyl-UTP: 10 µl
Storage
Store at -20°C
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