LiGreen™ Nucleic Acid Gel Stains
LiGreen™ is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. These stains are next-generation fluorescent nucleic acid gel stains designed to replace the highly mutagenic ethidium bromide (EB). These dyes are superior to EB by having a combination of high sensitivity, low toxicity and great stability.
| LiGreen™ | LiGreen™ Plus | LiGreen™ Ultra | LiGreen™ Red | |
| Sensitivity (dsDNA) | Sensitive (~2 ng) | Highly sensitive (~0.2 ng) | Ultra sensitive (~60 pg) | Sensitive (~1 ng) |
| Nonhazardous | √ | √ | √ | √ |
| dsDNA | √ | √ | √ | √ |
| ssDNA | √ | √ | ||
| RNA | √ | √ | ||
| Precast gel staining | √ | √ | √ | |
| Post-gel staining | √ | |||
| Gel band excision for cloning | √ | √ | √ | |
| Excitation Source | Blue light/UV | Blue light/UV | Blue light/UV | UV |
| Recommended emission filter | SYBR filter | SYBR filter | SYBR filter | EB filter |
Key Features
• Safer than EB: Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.
• Ultra-sensitive: Much more sensitive than GelGreen and SYBR Safe.
• Compatible with standard blue LED and UV transilluminators.
• Use with blue light reduces mutagenesis for gel excision and subsequent cloning.
| Product Name | Cat. # | Price | Order |
| LiGreen™ DNA Gel Stain (1 ml) | M0049 | $149 |  |
| LiGreen™ Plus Nucleic Acid Gel Stain (500 μl) | M0050 | $119 | |
| LiGreen™ Ultra Nucleic Acid Gel Stain (500 μl) | M0051 | $149 | |
| LiGreen™ Loading Dye (2× 1ml) | M0052 | $99 | |
| LiGreen™ Red DNA Gel Stain (500 µl) | M0054 | $109 | |
| LiGreen™ Red Loading Dye (2× 1ml) | M0055 | $99 | |
| SYBR Green I DNA Gel Stain (500 μl) | M0058 | $189 | |
| SYBR Green II RNA Gel Stain (500 μl) | M0092 | $189 | |
| SYBR Gold DNA Gel Stain (500 μl) | M0097 | $149 | |
| SYBR Safe DNA Gel Stain (400 μl) | M0098 | $69 | |
| 6× DNA Loading Buffer (5× 5ml) | M0099 | $99 | |
Case Study
Gel stain results with LiGreen™, LiGreen™ Plus, and LiGreen™ Ultra (Left), and comparison results with SYBR Safe and GelGreen (Right). Left: Two-fold serial dilutions of a 1 kb DNA ladder were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with LiGreen™, LiGreen™ Plus, and LiGreen™ Ultra. Right: Results show LiGreen™ has similar sensitivity with SYBR Safe and GelGreen, LiGreen™ Plus and LiGreen™ Ultra have much better sensitivity.
Cell permeability tests with SYBR Safe, LiGreen™, LiGreen™ Plus, and LiGreen™ Red. Hela cells were incubated at 37ºC with 1× SYBR Safe, LiGreen™, LiGreen™ Plus, and LiGreen™ Red for 30 min. LiGreen™ Plus and LiGreen™ Red could not penetrate cell membranes to bind DNA in living cells, and were much safer than SYBR Safe, which rapidly entered cells and stained nuclei.
