The LiClone™ technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning.

LiClone™ Ultra One-Step Cloning Kit is a new generation of recombinant cloning kit, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.
 

Key Features

• Simple: Ready-to-use super mix in one tube.

• Rapid: saves 2-3 hours by eliminating vector digestion and gel purification steps.

• High-efficiency: Typically >95% of colonies bear the correctly inserted DNA fragment.

• Compatible: Suitable for GC-rich/AT-rich homologous sequences.

• Special optimization: Maximum compatible with 15 - 35 bp overlaps, not only 15 - 20 bp.
 

Product Name	Cat. #	Price	View
LiClone™ One-Step Cloning Kit (20 rxns)	M0010	$119
LiClone™ Ultra One-Step Cloning Kit (20 rxns)	M0011	$219
LiClone™ Fast Mutagenesis Kit (10 rxns)	M0012	$169
LiClone™ Fast DNA Ligation Kit (30 rxns)	M0009	$99