LIFESCT offers 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, featuring our exceptionally bright and photostable LiFluor™ fluorescent dyes. Fluorescent and biotin-labeled dUTP and UTP are widely used for enzymatic incorporation into DNA or RNA.
 

Key Features

• Bright signals with low background.

• Wide variety of fluorophores.

• Great for many applications, like Fluorescence in situ Hybridization (FISH), TUNEL, and more.
 

Product Name	Cat. #	Price	View
5-Aminoallyl-dUTP (100 μl)	M0070	$129
5-Aminoallyl-UTP (100 μl)	M0071	$119
5-Ethynyl-dUTP (500 μl)	M0168	$199
Biotin-11-dUTP (50 μl)	M0072	$199
Biotin-11-UTP (50 μl)	M0073	$199
Biotin-14-dCTP (50 nmol)	M0074	$389
Biotin-14-dATP (50 nmol)	M0044	$399
5-FAM-dUTP (25 μl)	M0075	$149
5-FAM-X-dUTP (25 μl)	M0076	$199
5-FAM-UTP (25 μl)	M0077	$149
5-FAM-X-UTP (25 μl)	M0078	$199
LiFluor™ 405M-UTP (25 μl)	M0149	$299
LiFluor™ 488-X-dUTP (25 μl)	M0079	$299
LiFluor™ 488-X-UTP (25 μl)	M0080	$299
LiFluor™ 555-X-dUTP (25 μl)	M0081	$299
LiFluor™ 555-X-UTP (25 μl)	M0082	$299
LiFluor™ 568-X-dUTP (25 μl)	M0083	$299
LiFluor™ 568-X-UTP (25 μl)	M0084	$299
LiFluor™ 594-X-dUTP (25 μl)	M0085	$299
LiFluor™ 594-X-UTP (25 μl)	M0086	$299
LiFluor™ 647-X-dUTP (25 μl)	M0087	$299
LiFluor™ 647-X-UTP (25 μl)	M0088	$299
Cy3-X-dUTP (25 μl)	M0142	$259
Cy3-X-UTP (25 μl)	M0143	$259
Cy5-X-dUTP (25 μl)	M0144	$259
Cy5-X-UTP (25 μl)	M0145	$259

Case Study

Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with LiFluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with LiFluor 488-X-dUTP (green) and LiFluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with LiFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).